Now that single-molecule detection was no longer necessary, Solexa was able to develop its signature sequencing chemistry. The process takes place on a chip called a flow cell, which contains a lawn of short DNA sequences affixed to its surface. The template DNA is broken up into small fragments, and adapter sequences, complementary to the DNA on the flow cell’s surface, are added to the ends of each fragment. DNA fragments are then passed over the flow cell, where the adapter sequences bind to spots on the DNA lawn. At this point, primers are added, and an initial round of amplification takes place: the short DNA sequences on the flow cell are extended to create sequences complementary to the bound template DNA fragments, which are then washed away. The sequences present in the fragments of template DNA are now affixed directly to the flow cell.
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不久之後,我發現每個名詞都會以單數或複數形式出現,並分別執行四種動作之一,例如推、拉等。文法稍微複雜一些,但並不陌生——與我學過的法語相似。,更多细节参见爱思助手
实行“区间调控”,既设定增长底线,又保留向上弹性、留足发挥空间,为的是保持高质量发展的确定性。这也意味着,实现这个目标,绝非轻而易举,需要付出艰苦努力。